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fugw-perceval hr  (Addgene inc)


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    Structured Review

    Addgene inc fugw-perceval hr
    Reporter organoids with real-time biosensor <t>Perceval</t> HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.
    Fugw Perceval Hr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugw-perceval hr/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    fugw-perceval hr - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "ATP/ADP biosensor organoids for drug nephrotoxicity assessment"

    Article Title: ATP/ADP biosensor organoids for drug nephrotoxicity assessment

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2023.1138504

    Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.
    Figure Legend Snippet: Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.

    Techniques Used: Transformation Assay

    Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Detection of nephrotoxicity by live monitoring of ATP/ADP ratio in 2D reporter organoids treated with 2.5 μg/mL AA and 5 µM cisplatin. (B) Representative images of ATP/ADP ratio monitoring in minimized 3D reporter organoids cultured on 384-well plates. The organoids were exposed to various toxicants (2.5 μg/mL AA, 5 or 50 µM cisplatin).
    Figure Legend Snippet: Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Detection of nephrotoxicity by live monitoring of ATP/ADP ratio in 2D reporter organoids treated with 2.5 μg/mL AA and 5 µM cisplatin. (B) Representative images of ATP/ADP ratio monitoring in minimized 3D reporter organoids cultured on 384-well plates. The organoids were exposed to various toxicants (2.5 μg/mL AA, 5 or 50 µM cisplatin).

    Techniques Used: Cell Culture

    Evaluation of the segment-specific injury by Perceval HR organoids. (A) Representative BF image and IF image of PODXL (red), LTL (cyan), and CDH1 (yellow) in 2D organoids. Scale bars: 200 μm. (B) Representative images for quantification of ATP/ADP ratio matched in position with the BF and IF images for segment identification in 2D organoids exposed to 5 μM cisplatin. Scale bars: 200 μm. (C) Detection of ATP/ADP ratio of each segment in 2D reporter organoids treated with hypoglucose, 5 and 50 μM cisplatin for 24 h n = 4 to 15. * p < 0.05.
    Figure Legend Snippet: Evaluation of the segment-specific injury by Perceval HR organoids. (A) Representative BF image and IF image of PODXL (red), LTL (cyan), and CDH1 (yellow) in 2D organoids. Scale bars: 200 μm. (B) Representative images for quantification of ATP/ADP ratio matched in position with the BF and IF images for segment identification in 2D organoids exposed to 5 μM cisplatin. Scale bars: 200 μm. (C) Detection of ATP/ADP ratio of each segment in 2D reporter organoids treated with hypoglucose, 5 and 50 μM cisplatin for 24 h n = 4 to 15. * p < 0.05.

    Techniques Used:



    Similar Products

    fugw-perceval hr  (Addgene inc)
    90
    Addgene inc fugw-perceval hr
    Reporter organoids with real-time biosensor <t>Perceval</t> HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.
    Fugw Perceval Hr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fugw-perceval hr/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    fugw-perceval hr - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: ATP/ADP biosensor organoids for drug nephrotoxicity assessment

    doi: 10.3389/fcell.2023.1138504

    Figure Lengend Snippet: Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Bright-field (B.F.) and ATP/ADP ratio (lower) in nephron-like structures of 2D reporter organoids. (B) The channels of ATP (ex495/em540) and ADP (ex436/em540) are used for ratio calculation, transformed in artificial colors, and merged with the bright field. (C) Signal intensities at varied excitation wavelengths from 400 to 500 nm in the parental H9 organoids and Perceval H9 organoids in the normal and hypoglucose culture. (D) The signal ratios of em540 excited by 490 and 430 in the control and hypoglucose organoids, evaluated by a plate reader. The parental H9 was used as a control. (E) The reactivity of ATP/ADP ratio to toxic substance using NaN3. NaN3 was added at 0 min, and the same media were kept in the wells until 45 min. (F) 2D Reporter organoids before freezing, just after thawing, and 1 day after thawing.

    Article Snippet: Lentivirus carrying Perceval HR was produced in HEK293 cells by transfection of FUGW-Perceval HR (addgene) ( ) and was transduced to H9.

    Techniques: Transformation Assay

    Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Detection of nephrotoxicity by live monitoring of ATP/ADP ratio in 2D reporter organoids treated with 2.5 μg/mL AA and 5 µM cisplatin. (B) Representative images of ATP/ADP ratio monitoring in minimized 3D reporter organoids cultured on 384-well plates. The organoids were exposed to various toxicants (2.5 μg/mL AA, 5 or 50 µM cisplatin).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: ATP/ADP biosensor organoids for drug nephrotoxicity assessment

    doi: 10.3389/fcell.2023.1138504

    Figure Lengend Snippet: Reporter organoids with real-time biosensor Perceval HR to detect ATP/ADP ratio. (A) Detection of nephrotoxicity by live monitoring of ATP/ADP ratio in 2D reporter organoids treated with 2.5 μg/mL AA and 5 µM cisplatin. (B) Representative images of ATP/ADP ratio monitoring in minimized 3D reporter organoids cultured on 384-well plates. The organoids were exposed to various toxicants (2.5 μg/mL AA, 5 or 50 µM cisplatin).

    Article Snippet: Lentivirus carrying Perceval HR was produced in HEK293 cells by transfection of FUGW-Perceval HR (addgene) ( ) and was transduced to H9.

    Techniques: Cell Culture

    Evaluation of the segment-specific injury by Perceval HR organoids. (A) Representative BF image and IF image of PODXL (red), LTL (cyan), and CDH1 (yellow) in 2D organoids. Scale bars: 200 μm. (B) Representative images for quantification of ATP/ADP ratio matched in position with the BF and IF images for segment identification in 2D organoids exposed to 5 μM cisplatin. Scale bars: 200 μm. (C) Detection of ATP/ADP ratio of each segment in 2D reporter organoids treated with hypoglucose, 5 and 50 μM cisplatin for 24 h n = 4 to 15. * p < 0.05.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: ATP/ADP biosensor organoids for drug nephrotoxicity assessment

    doi: 10.3389/fcell.2023.1138504

    Figure Lengend Snippet: Evaluation of the segment-specific injury by Perceval HR organoids. (A) Representative BF image and IF image of PODXL (red), LTL (cyan), and CDH1 (yellow) in 2D organoids. Scale bars: 200 μm. (B) Representative images for quantification of ATP/ADP ratio matched in position with the BF and IF images for segment identification in 2D organoids exposed to 5 μM cisplatin. Scale bars: 200 μm. (C) Detection of ATP/ADP ratio of each segment in 2D reporter organoids treated with hypoglucose, 5 and 50 μM cisplatin for 24 h n = 4 to 15. * p < 0.05.

    Article Snippet: Lentivirus carrying Perceval HR was produced in HEK293 cells by transfection of FUGW-Perceval HR (addgene) ( ) and was transduced to H9.

    Techniques: